Dibromobimane 3,5-Bis-bromomethyl-2,6-dimethyl-pyrazolo[1,2-a]pyrazole-1,7-dione CAS: 68654-25-1 MDL: MFCD00036952
Molecular weight: 350.01 g/mol
Molecular Formula: C10H10Br2N2O2
CAS Number: 68654-25-1
Storage: Store at 2-8 Co, under dry conditions.
Synonyms: Dibromobimane, 68654-25-1, dBBr, bBBr, syn-(bromomethyl,methyl)bimane, CHEBI:62827, CHEMBL2158899
Uses: Nitrogen heterocycle, thiol reactive reagent, protein labelling, active site labels, thiol reactive, active site mapping
Dibromobimane, is a synthetic fine chemical useful for studying the biochemistry of macromolecules.
Selected References:
1.) Kosower, Nechama S., and Edward M. Kosower. Thiol Labeling with Bromobimanes. Methods in Enzymology 143, (1987): 76–84.
2.) Kosower, Nechama S., Edward M. Kosower, Gerald L. Newton, and Helen M. Ranney. Bimane Fluorescent Labels: Labeling of Normal Human Red Cells under Physiological Conditions. Proceedings of the National Academy of Sciences of the United States of America 76, (1979): 3382–86. https://doi.org/10.1073/pnas.76.7.3382.
3.) Loo, Tip W., and David M. Clarke. Identification of Residues in the Drug-Binding Domain of Human P-Glycoprotein. Analysis of Transmembrane Segment 11 by Cysteine-Scanning Mutagenesis and Inhibition by Dibromobimane. Journal of Biological Chemistry 274, (1999): 35388–92. https://doi.org/10.1074/jbc.274.50.35388.
4.) Identification of Residues in the Drug-Binding Site of Human P-Glycoprotein Using a Thiol-Reactive Substrate. Journal of Biological Chemistry 272, (1997): 31945–48. https://doi.org/10.1074/jbc.272.51.31945.
5.) Identification of Residues within the Drug-Binding Domain of the Human Multidrug Resistance P-Glycoprotein by Cysteine-Scanning Mutagenesis and Reaction with Dibromobimane. Journal of Biological Chemistry 275, (2000): 39272–78. https://doi.org/10.1074/jbc.M007741200.
6.) Location of the Rhodamine-Binding Site in the Human Multidrug Resistance P-Glycoprotein. The Journal of Biological Chemistry 277, no. Copyright (C) 2021 U.S. National Library of Medicine. (2002): 44332–38
7.) Location of the Rhodamine-Binding Site in the Human Multidrug Resistance P-Glycoprotein. Journal of Biological Chemistry 277, (2002): 44332–38. https://doi.org/10.1074/jbc.M208433200.
8.) Sinz, Andrea, and Kuan. Wang. Mapping Protein Interfaces with a Fluorogenic Cross-Linker and Mass Spectrometry: Application to Nebulin-Calmodulin Complexes. Biochemistry 40, (2001): 7903–13. https://doi.org/10.1021/bi010259+.
